Fig 2. Comparison of M. lucifugus gene expression in tissues from torpid and aroused bats either uninfected or infected with P. destructans.
(a) Differential expression between tissue infected with P. destructans and uninfected tissue in torpid bats. (b) Differential expression between tissue infected with P. destructans and uninfected tissue in bats after arousal. (c) Differential expression between tissue from torpid and aroused bats not infected with P. destructans. (d) Differential expression between tissue from torpid and aroused bats infected with P. destructans. (a-d) Expression of M. lucifugus genes is compared by edgeR with MA plots. The mean expression level (log2 counts per million (CPM)) and the fold change (log2 FC) are shown for each gene. Blue points indicate differential expression (FDR ≤ 0.05 determined by edgeR) for genes more highly expressed in the first comparison group and red genes are more highly expressed in the second group. Interactive versions of these graphs are available on the Bucknell Digital Archive. After opening the html file in a web browser, hover over each point to view the annotation metadata for that gene and the expression level (in log2CPM) for each sample. Individual genes can also be found by searching, for example by entering ENSMLUG00000008204 in the search box. (e) Multidimensional scaling plot analysis of global M. lucifugus gene expression using moderated log CPM expression levels. Points that are closer together are more similar. Points are colored by group, UV-negative torpid samples are blue, UV-negative euthermic samples are purple, UV-positive torpid samples are red, and UV-positive euthermic samples are green. (f) Venn diagram showing the differentially expressed genes in each pairwise comparison with FDR < 0.05 determined by edgeR. Areas of overlap represent genes differentially expressed in multiple categories. Table S2 lists the identity of the genes in each sector of the diagram.
Field, Kenneth A., "Figure 2d: Effect of torpor on host transcriptomic responses to a fungal pathogen in hibernating bats" (2018). Other Faculty Research and Publications. 127.