Crystallization, optimization and preliminary X-ray characterization of a metal-dependent PI-PLC from Streptomyces antibioticus
Publication Date
2012
Description
A recombinant metal-dependent phosphatidylinositol-specific phospholipase C (PI-PLC) from Streptomyces antibioticus has been crystallized by the hanging-drop method with and without heavy metals. The native crystals belonged to the orthorhombic space group P222, with unit-cell parameters a = 41.26, b = 51.86, c= 154.78 A. The X-ray diffraction results showed significant differences in the crystal quality of samples soaked with heavy atoms. Additionally, drop pinning, which increases the surface area of the drops, was also used to improve crystal growth and quality. The combination of heavy-metal soaks and drop pinning was found to be critical for producing high-quality crystals that diffracted to 1.23 A resolution.
Journal
Acta crystallographica. Section F, Structural biology and crystallization communications
Volume
68
Issue
11
First Page
1378
Last Page
1386
Department
Chemistry
Link to Published Version
Recommended Citation
Jackson, Michael R. and Selby, Thomas L.. "Crystallization, optimization and preliminary X-ray characterization of a metal-dependent PI-PLC from Streptomyces antibioticus." Acta crystallographica. Section F, Structural biology and crystallization communications (2012) : 1378-1386.