Title

Analysis of Dna Sequences From Largemouth Bass Virus Isolates From Smallmouth Bass

Item Type

Poster

Location

Elaine Langone Center, Terrace Room

Session

Poster Presentations

Start Date

21-11-2014 8:00 PM

End Date

21-11-2014 10:00 PM

Description

The viral pathogen, Largemouth Bass Virus (LMBV), is a member of the family Iridoviridae and has been known to cause large fish kills among Largemouth Bass, Micropterus salmoides. The viral pathogen is also consistently isolated from other species where diseased fish are found. Throughout the Susquehanna River Basin since 2005, Smallmouth Bass, Micropterus dolomieu, have been suffering from wide-spread disease related deaths. Even though LMBV is not clinically known to affect Smallmouth Bass, this virus is prevalent among diseased fish infected with other pathogens such as Pseudomonas aeruginosa. The question we seek to address is whether there are significant genetic differences between LMBV isolates from smallmouth bass and largemouth bass. Total DNA was isolated from preparations of LMBV derived from smallmouth bass and largemouth bass isolates. Five LMBV-specific primer sets were used to amplify fragments from each preparation for conventional Sanger sequencing and the total DNA preparations are also being analyzed using MiSeq NextGen sequence analysis. After we receive the sequence data, further assembly, annotation, and analysis will be conducted to identify any host-specific variations.

Language

eng

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Nov 21st, 8:00 PM Nov 21st, 10:00 PM

Analysis of Dna Sequences From Largemouth Bass Virus Isolates From Smallmouth Bass

Elaine Langone Center, Terrace Room

The viral pathogen, Largemouth Bass Virus (LMBV), is a member of the family Iridoviridae and has been known to cause large fish kills among Largemouth Bass, Micropterus salmoides. The viral pathogen is also consistently isolated from other species where diseased fish are found. Throughout the Susquehanna River Basin since 2005, Smallmouth Bass, Micropterus dolomieu, have been suffering from wide-spread disease related deaths. Even though LMBV is not clinically known to affect Smallmouth Bass, this virus is prevalent among diseased fish infected with other pathogens such as Pseudomonas aeruginosa. The question we seek to address is whether there are significant genetic differences between LMBV isolates from smallmouth bass and largemouth bass. Total DNA was isolated from preparations of LMBV derived from smallmouth bass and largemouth bass isolates. Five LMBV-specific primer sets were used to amplify fragments from each preparation for conventional Sanger sequencing and the total DNA preparations are also being analyzed using MiSeq NextGen sequence analysis. After we receive the sequence data, further assembly, annotation, and analysis will be conducted to identify any host-specific variations.