Date of Thesis

2009

Thesis Type

Honors Thesis

Department

Biology

First Advisor

Marie C. Pizzorno

Keywords

bees, virus, honeybees, European honeybees, deformed wing virus, DWV, CCD, colony collpase disorder, in-situ-hybridization, real-time PCR, bee brains, bee viruses, virus causing behavioral changes

Abstract

The purpose of the current research project is to design a successful in-situ hybridization to identify regions within the brains of honeybees where DWV replicates. The localization of the virus in the brains of the bees can draw a connection between CCDand DWV.In conclusion, these results demonstrate that in bees infected with DWV the virus replicates actively in very important regions of the brain, including neuropils that are responsible for vision and olfaction. This means that the virus could adversely affect the vision and olfaction of the honeybees making it difficult for bees to behave normally.

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